Quickly attain sections as slender or thick as your software calls for, with no worrying about losing or distorting important tissue samples.
Megatome is actually a vibrating microtome designed to part a broad number of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With significant blade vibrating frequency and minimized blade deflection, Megatome allows high-throughput tissue sectioning with uniform area profile, and also minimal tissue problems and information decline.
Antibodies could choose weeks to diffuse as a result of just a few millimeters of tissue, using a steep labeling gradient from area to core.
Megatome is suitable for accuracy: the blade vibrates at a better frequency and bigger amplitude assortment than other microtomes, and encompasses a special deflection Regulate mechanism.
SE utilizes a rotational electrical field to disperse very electromobile molecules (which include antibodies or surfactant micelles) during a porous sample devoid of damaging electrically charged buildings inside the tissue. This enables two-four working day clearing of intact organs,
Our preformulated EasyIndex Resolution raises and homogenize the refractive index of delipidated tissue samples, rendering them entirely transparent. This enables mild penetration in the sample and makes sure the acquisition of superior-resolution, in-concentrate graphic facts.
Megatome is often a novel microtome which allows for high-precision sectioning of a wide range of tissue samples – from organoids, to arrays of animal organs, to intact human brain hemispheres – with minimal tissue damage and information loss.
It’s the perfect time to enhance your microtome to Megatome. With exact superior-frequency slicing for an unmatched number of sample dimensions and kinds – from organoids and tumors to expanded tissues, sample arrays, and intact primate organs – Megatome is optimized for varied programs.
The SmartSPIM mild sheet microscope gives unparalleled resolution and acquisition speed all through intact tissue volumes, whilst its modular style enables you to improve and customize individual components.
Docset creation requires at minimum amount two options: megatomi.com the name of the docset and The situation of the Javadoc data files to incorporate from the docset.
This tends to develop a docset named Sample in The present directory. Docset creation might be custom-made with optional arguments:
Possibly obtain a release or create a distribution zip as outlined above. Unzip the archive to a wished-for site.
Leverage the Obvious+ tissue clearing technique, along with eFLASH and patented stochastic electrotransport systems, to swiftly clear and label full organs. Essential highlights and attributes involve:
Our novel Defend tissue preservation approach sorts intramolecular bonds working with polyfunctional, flexible epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.